Polyphenol-Based Nanoparticles for Intracellular Protein Delivery via Competing Supramolecular Interactions
Yiyuan Han, Jiajing Zhou, Yingjie Hu, Zhixing Lin, Yutian Ma, Joseph J Richardson, Frank Caruso
ACS Nano | AMER CHEMICAL SOC | Published : 2020
Intracellular delivery of proteins is a promising strategy for regulating cellular behavior and therefore has attracted interest for biomedical applications. Despite the emergence of various nanoparticle-based intracellular delivery approaches, it remains challenging to engineer a versatile delivery system capable of responding to various physiological triggers without the need for complex chemical synthesis of the delivery system. Herein, we develop a template-mediated supramolecular assembly strategy to synthesize protein-polyphenol nanoparticles (NPs) capable of endosomal escape and subsequent protein release in the cytosol. These NPs are stable in serum and undergo surface charge reversa..View full abstract
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Awarded by Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology
Awarded by National Health and Medical Research Council Senior Principal Research Fellowship
This research was conducted and funded by the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (Project Number CE140100036). F.C. acknowledges the award of a National Health and Medical Research Council Senior Principal Research Fellowship (GNT1135806). This work was performed in part at the Materials Characterization and Fabrication Platform and the Bio21 Advanced Microscopy Facility at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility. We thank Dr. Rene Lafleur, Dr. Christina Cortez-Jugo, Denzil Furtado, and Agata Glab for helpful discussions and Paula Cevaal, Dr. Yi Ju, Jingqu Chen, Yijiao Qu, and Jiaying Song for assistance with experiments.