Journal article

Long Chain Fatty Acids Differentially Regulate Sub-populations of Arcuate POMC and NPY Neurons

Natalie J Michael, Matthew J Watt

Neuroscience | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2020


Long chain fatty acids (LCFAs) have been suggested to influence the activity of hypothalamic neurons, however, limited studies have attempted to identify the neurochemical phenotype of these neurons. We aimed to determine if physiological levels of LCFAs alter the electrical excitability of pro-opiomelanocortin (POMC) and neuropeptide Y (NPY) neurons in the arcuate nucleus of the hypothalamus. We utilised whole-cell patch-clamp electrophysiology on brain slice preparations from genetic mouse models where green fluorescent protein was expressed in either POMC or NPY expressing cells. All animals had undergone an overnight fast to replicate conditions in which fatty acids would usually increas..

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University of Melbourne Researchers


Awarded by National Health and Medical Research Council of Australia

Awarded by NHMRC

Funding Acknowledgements

We thank the National Health and Medical Research Council of Australia for its support (NHMRC, ID: 1079233). MJW is supported by a Senior Research Fellowship from the NHMRC (ID: 1077703). We also thank Dr. Michael Cowley (Monash University) for his generous donation of the mice; Dr. Fei-Yue Zhao (NeurosolutionsLtd) for technical assistance; and Dr. Alexandre Caron (Universite ' Laval) for critically reviewing the manuscript.