Advanced glycation end products present in the obese uterine environment compromise preimplantation embryo development
Jennifer C Hutchison, Thi T Truong, Lois A Salamonsen, David K Gardner, Jemma Evans
Reproductive BioMedicine Online | ELSEVIER SCI LTD | Published : 2020
RESEARCH QUESTION: Proinflammatory advanced glycation end products (AGE), highly elevated within the uterine cavity of obese women, compromise endometrial function. Do AGE also impact preimplantation embryo development and function? DESIGN: Mouse embryos were cultured in AGE equimolar to uterine fluid concentrations in lean (1-2 µmol/l) or obese (4-8 µmol/l) women. Differential nuclear staining identified cell allocation to inner cell mass (ICM) and trophectoderm (TE) (day 4 and 5 of culture). Cell apoptosis was examined by terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling assay (day 5). Day 4 embryos were placed on bovine serum albumin/fibronectin-coated plates and embr..View full abstract
The authors acknowledge Ms Kathryn Gurner for her technical assistance, and Professor Melinda Coughlan for quantification of AGE-HSA. This work was supported in part by: a bridge grant from the Study for Reproductive Investigation (to JE); the Victorian Government Operational Infrastructure support program to the Hudson Institute; University of Melbourne (to TTT and DKG); Australian Government Research Training Program (RTP) Scholarship (to JH); Fielding Foundation fellowship (to JE).