Journal article

Detection of naturally aerosolized Actinobacillus pleuropneumoniae on pig farms by cyclonic air sampling and qPCR

Anne E Watt, Glenn F Browning, Philip F Markham, Marc S Marenda

Veterinary Microbiology | ELSEVIER | Published : 2020


Respiratory infections caused by Actinobacillus pleuropneumoniae have a large impact on commercial pig farms globally. As current vaccines have limited efficacy, animal care and air hygiene are critical for disease control. Here we used a Coriolis μ cyclonic air sampler and an A. pleuropneumoniae-specific apxIV gene qPCR assay to detect the organism. Air samples were collected into a liquid medium by the Coriolis μ sampler for A. pleuropneumoniae detection by plate culture and qPCR assay. The method was validated by comparing the Coriolis μ sampler and a plate impactor (Millipore Air-T) in a specially designed aerosolization chamber. Two commercial farms, housing pigs between 3 and 21 weeks ..

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