Journal article

Aldehyde Production as a Calibrant of Ultrasonic Power Delivery During Protein Misfolding Cyclic Amplification

Simon C Drew

The Protein Journal | SPRINGER | Published : 2020


The protein misfolding cyclic amplification (PMCA) technique employs repeated cycles of incubation and sonication to amplify minute amounts of misfolded protein conformers. Spontaneous (de novo) prion formation and ultrasonic power level represent two potentially interrelated sources of variation that frustrate attempts to replicate results from different laboratories. We previously established that water splitting during PMCA provides a radical-rich environment leading to oxidative damage to substrate molecules as well as the polypropylene PCR tubes used for sample containment. Here it is shown that the cross-linking agent formaldehyde is generated from buffer ions that are attacked by hydr..

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Funding Acknowledgements

This work was supported by a grant awarded by the Australian Creutzfeldt-Jakob Disease Support Group Network (CJDSGN) in memory of Silva Coelho, and a grant awarded by the CJDSGN in memory of Frank Burton, Bassil Gianniodis, Primo Monaci, Rhonda Sanders, Cesarina Stilla, Ross Glasscock and others lost to CJD.