Intranasal Delivery of a Chitosan-Hydrogel Vaccine Generates Nasal Tissue Resident Memory CD8( ) T Cells That Are Protective against Influenza Virus Infection
James G Bedford, Irina Caminschi, Linda M Wakim
Vaccines | MDPI | Published : 2020
Rapid antigen clearance from the nasal mucosa is one of the major challenges in the development of intranasal vaccines. Here, we tested whether intranasal immunization with a chitosan-hydrogel vaccine, with in situ gelling properties, extended antigen retention time within the nasal mucosa. Intranasal immunization with a chitosan-hydrogel vaccine retained antigen within the upper respiratory tract (URT), while intranasal delivery of less viscous vaccines led to antigen accumulation within the lower airways. Interestingly, sustained antigen retention within the URT following chitosan-hydrogel vaccination boosted the number of vaccine-specific, tissue resident memory (Trm) CD8+ T cells that de..View full abstract
Awarded by National Health and Medical Research Council of Australia
This research was funded by the National Health and Medical Research Council of Australia (L.M.W.). Code: APP1143735, APP1120270. IC also supported by NHMRC-APP1124706.