Lipid A profiling and metabolomics analysis of paired polymyxin-susceptible and -resistant MDR Klebsiella pneumoniae clinical isolates from the same patients before and after colistin treatment
Su Mon Aye, Irene Galani, Mei-Ling Han, Ilias Karaiskos, Darren J Creek, Yan Zhu, Yu-Wei Lin, Tony Velkov, Helen Giamarellou, Jian Li
Journal of Antimicrobial Chemotherapy | OXFORD UNIV PRESS | Published : 2020
BACKGROUND: The increased incidence of polymyxin-resistant MDR Klebsiella pneumoniae has become a major global health concern. OBJECTIVES: To characterize the lipid A profiles and metabolome differences between paired polymyxin-susceptible and -resistant MDR K. pneumoniae clinical isolates. METHODS: Three pairs of K. pneumoniae clinical isolates from the same patients were examined [ATH 7 (polymyxin B MIC 0.25 mg/L) versus ATH 8 (64 mg/L); ATH 15 (0.5 mg/L) versus ATH 16 (32 mg/L); and ATH 17 (0.5 mg/L) versus ATH 18 (64 mg/L)]. Lipid A and metabolomes were analysed using LC-MS and bioinformatic analysis was conducted. RESULTS: The predominant species of lipid A in all three paired isolates ..View full abstract
Awarded by National Institute of Allergy and Infectious Diseases of the National Institutes of Health
This study was supported by an internal fund and a research grant from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (R01 AI132681, J.L. and T.V.). D.J.C. and T.V. are also supported by the Australian National Health and Medical Research Council (NHMRC) as Principal Research Fellow (J.L.) and Career Development Level 2 Fellows (T.V. and D.J.C.).