Journal article

Elucidation of Proteus mirabilis as a Key Bacterium in Crohn's Disease Inflammation

Jingwan Zhang, Emily C Hoedt, Qin Liu, Erwin Berendsen, Jing Jie Teh, Amy Hamilton, Amy Wilson O' Brien, Jessica YL Ching, Hong Wei, Keli Yang, Zhilu Xu, Sunny H Wong, Joyce WY Mak, Joseph JY Sung, Mark Morrison, Jun Yu, Michael A Kamm, Siew C Ng

Gastroenterology | W B SAUNDERS CO-ELSEVIER INC | Published : 2021

Abstract

BACKGROUND & AIMS: Proteus spp, Gram-negative facultative anaerobic bacilli, have recently been associated with Crohn's disease (CD) recurrence after intestinal resection. We investigated the genomic and functional role of Proteus as a gut pathogen in CD. METHODS: Proteus spp abundance was assessed by ure gene-specific polymerase chain in 54 pairs of fecal samples and 101 intestinal biopsies from patients with CD and healthy controls. The adherence, invasion, and intracellular presence of 2 distinct isolates of Proteus mirabilis in epithelial cells were evaluated using immunofluorescence and electron microscopy. Intracellular gene expression profiles and regulated pathways were analyzed by R..

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Grants

Funding Acknowledgements

Supported by The Leona M. and Harry B. Helmsley Charitable Trust and Croucher Senior Medical Research Fellowship. The Translational Research Institute is funded by a grant from the Australian Federal Government.