Elucidation of Proteus mirabilis as a Key Bacterium in Crohn's Disease Inflammation
Jingwan Zhang, Emily C Hoedt, Qin Liu, Erwin Berendsen, Jing Jie Teh, Amy Hamilton, Amy Wilson O' Brien, Jessica YL Ching, Hong Wei, Keli Yang, Zhilu Xu, Sunny H Wong, Joyce WY Mak, Joseph JY Sung, Mark Morrison, Jun Yu, Michael A Kamm, Siew C Ng
Gastroenterology | W B SAUNDERS CO-ELSEVIER INC | Published : 2021
BACKGROUND & AIMS: Proteus spp, Gram-negative facultative anaerobic bacilli, have recently been associated with Crohn's disease (CD) recurrence after intestinal resection. We investigated the genomic and functional role of Proteus as a gut pathogen in CD. METHODS: Proteus spp abundance was assessed by ure gene-specific polymerase chain in 54 pairs of fecal samples and 101 intestinal biopsies from patients with CD and healthy controls. The adherence, invasion, and intracellular presence of 2 distinct isolates of Proteus mirabilis in epithelial cells were evaluated using immunofluorescence and electron microscopy. Intracellular gene expression profiles and regulated pathways were analyzed by R..View full abstract
Supported by The Leona M. and Harry B. Helmsley Charitable Trust and Croucher Senior Medical Research Fellowship. The Translational Research Institute is funded by a grant from the Australian Federal Government.