Experimentally Guided Computational Methods Yield Highly Accurate Insights into Transmembrane Interactions within the T Cell Receptor Complex
Samyuktha Ramesh, Soohyung Park, Melissa J Call, Wonpil Im, Matthew E Call
The Journal of Physical Chemistry B | AMER CHEMICAL SOC | Published : 2020
Understanding how molecular interactions within the plasma membrane govern assembly, clustering, and conformational changes in single-pass transmembrane (TM) receptors has long presented substantial experimental challenges. Our previous work on activating immune receptors has combined direct biochemical and biophysical characterizations with both independent and experimentally restrained computational methods to provide novel insights into the key TM interactions underpinning assembly and stability of complex, multisubunit receptor systems. The recently published cryo-EM structure of the intact T cell receptor (TCR)-CD3 complex provides a unique opportunity to test the models and predictions..View full abstract
Awarded by NSF
Awarded by NHMRC
This work was supported in part by grants from the NSF, MCB-1810695 (WI) and NHMRC GNT1011352 (M.E.C., M.J.C.). The authors thank the many researchers who contributed to the experimental and computational work described herein.