Journal article
Human yolk sac-like haematopoiesis generates RUNX1-, GFI1- and/or GFI1B-dependent blood and SOX17-positive endothelium
Freya F Bruveris, Elizabeth S Ng, Ana Rita Leitoguinho, Ali Motazedian, Katerina Vlahos, Koula Sourris, Robyn Mayberry, Penelope McDonald, Lisa Azzola, Nadia M Davidson, Alicia Oshlack, Edouard G Stanley, Andrew G Elefanty
Development | Company Biologists Ltd | Published : 2020
DOI: 10.1242/dev.193037
Abstract
The genetic regulatory network controlling early fate choices during human blood cell development are not well understood. We used human pluripotent stem cell reporter lines to track the development of endothelial and haematopoietic populations in an in vitro model of human yolk-sac development. We identified SOX17−CD34+CD43− endothelial cells at day 2 of blast colony development, as a haemangioblast-like branch point from which SOX17−CD34+CD43+ blood cells and SOX17+CD34+CD43− endothelium subsequently arose. Most human blood cell development was dependent on RUNX1. Deletion of RUNX1 only permitted a single wave of yolk sac-like primitive erythropoiesis, but no yolk sac myelopoiesis or aorta..
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Awarded by National Health and Medical Research Council of Australia
Funding Acknowledgements
This study was funded by the National Health and Medical Research Council of Australia through research fellowships awarded to A.G.E. (GNT1117596) and E.G. S. (GNT1079004), and through project grants awarded to A.G.E. and E.G. S. (GNT1068866 and GNT1129861); by the Australian Research Council Special Research Initiative in Stem Cells (Stem Cells Australia) and by the Stafford Fox Medical Research Foundation. Additional infrastructure funding to the Murdoch Children's Research Institute was provided by the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme and the Victorian Government's Operational Infrastructure Support Program. Deposited in PMC for immediate release.