Estradiol Enhances Antiviral CD4( ) Tissue-Resident Memory T Cell Responses following Mucosal Herpes Simplex Virus 2 Vaccination through an IL-17-Mediated Pathway
Puja Bagri, Ramtin Ghasemi, Joshua JC McGrath, Danya Thayaparan, Emma Yu, Andrew G Brooks, Martin R Staempfli, Charu Kaushic
Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2021
Estradiol (E2) is a sex hormone which has been shown to be protective against sexually transmitted infections such as herpes simplex virus 2 (HSV-2). However, few studies have examined the underlying mechanisms by which this occurs. Here, we investigated the effect of E2 on the establishment of memory T cells post-intranasal immunization with HSV-2. CD4+ T cell responses first appeared in the upper respiratory tract (URT) within 3 days postimmunization before being detected in the female reproductive tract (FRT) at 7 days. E2 treatment resulted in greater and earlier Th17 responses, which preceded augmented Th1 responses at these sites. The CD4+ T cells persisted in the URT for up to 28 days..View full abstract
Awarded by Canadian Institutes of Health Research (CIHR)
Awarded by Ontario HIV Treatment Network (OHTN) (AHRC)
P.B. and C.K. conceived and designed the experiments. P.B., R.G., J.J.C.M., D.T., and E.Y. performed the experiments. P.B. and C.K. analyzed the data. A.G.B. and M.R.S. provided materials and assisted with data analysis. P.B. and C.K. wrote the manuscript. C.K. supervised the work and obtained funding. We thank Hong Liang and Varun Anipindi for providing technical assistance. This study was supported by operating grants from the Canadian Institutes of Health Research (CIHR) to C.K. (FRN no. 93615) and an Applied HIV Research Chair Award to C.K. from the Ontario HIV Treatment Network (OHTN) (AHRC no. 779). P.B. was supported by the CIHR Frederick Banting and Charles Best Canada Graduate Scholarship. The funders had no role in the design of the study, data collection and analysis, or the decision to submit the work for publication. We declare no conflict of interest.