RYK-mediated filopodial pathfinding facilitates midgut elongation
Sha Wang, James P Roy, Abigail J Tomlinson, Ellen B Wang, Yu-Hwai Tsai, Lisa Cameron, Julie Underwood, Jason R Spence, Katherine D Walton, Steven A Stacker, Deborah L Gumucio, Terry Lechler
DEVELOPMENT | COMPANY BIOLOGISTS LTD | Published : 2020
Between embryonic days 10.5 and 14.5, active proliferation drives rapid elongation of the murine midgut epithelial tube. Within this pseudostratified epithelium, nuclei synthesize DNA near the basal surface and move apically to divide. After mitosis, the majority of daughter cells extend a long, basally oriented filopodial protrusion, building a de novo path along which their nuclei can return to the basal side. WNT5A, which is secreted by surrounding mesenchymal cells, acts as a guidance cue to orchestrate this epithelial pathfinding behavior, but how this signal is received by epithelial cells is unknown. Here, we have investigated two known WNT5A receptors: ROR2 and RYK. We found that epi..View full abstract
Awarded by National Institutes of Health
This work was supported by the National Institutes of Health (R01 DK089933 to D.L.G. and R01 DK117981 to T.L.). Deposited in PMC for release after 12 months.