Journal article

Skeletal Phenotype and Mechanisms of Bone Loss in Winnie Mice as a Model for Inflammatory Bowel Disease

Ahmed Al Saedi, Shilpa Sharma, Ebrahim Bani Hassan, Lulu Chen, Ali Ghasem-Zadeh, Majid Hassanzadeganroudsari, Jonathan Gooi, Rhian Stavely, Rajaraman Eri, Dengshun Miao, Kulmira Nurgali, Gustavo Duque

Published : 2020


Objective We aimed to investigate the skeletal phenotype of Winnie mouse model of spontaneous chronic colitis, which carries a mutation in the Muc2 gene and closely replicates IBD symptoms and pathophysiology. These mice have a high level of gut-derived serotonin (GDS), a potent osteoblastogenesis inhibitor. We explored the underlying mechanisms of bone loss associated with chronic intestinal inflammation. Design Winnie male and female mice prior to colitis onset (6 weeks old) and progression (14 and 24 weeks) were compared to age- and sex-matched C57BL/6 controls. We assessed bone quality (static and dynamic histomorphometry, micro-CT, 3-point bending), intestinal inflammation (lipocalin-2)..

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