Peripheral T helper cell profiles during management of periodontitis
Nidhi Medara, Jason C Lenzo, Katrina A Walsh, Neil M O'Brien-Simpson, Eric C Reynolds, Ivan B Darby
JOURNAL OF CLINICAL PERIODONTOLOGY | WILEY | Published : 2020
AIM: Periodontitis has been associated with other systemic diseases with underlying inflammation responsible for the shared link. This study evaluated longitudinal variation in peripheral T helper cells in periodontitis patients undergoing management over 1 year. MATERIALS AND METHODS: Periodontal parameters and peripheral blood mononuclear cells (PBMCs) were collected from 54 periodontitis patients at baseline, and 3-, 6- and 12-months post-treatment and 40 healthy controls. IFN-γ+ , IL-4+ , IL-17+ and Foxp3+ and their double-positive expression were identified in CD4+ and TCRαβ+ cells using flow cytometry. PBMCs were incubated with P. gingivalis, and IFN-γ, IL-4, IL-17 and IL-10 in cell su..View full abstract
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Awarded by National Health and Medical Research Council
Awarded by Australian Dental Research Foundation (ADRF)
This study was supported by grants from the National Health and Medical Research Council (APP1029878) and the Australian Dental Research Foundation (ADRF, 211-2017).