The effect of high-fat diet-induced metabolic disturbance on corneal neuroimmune features
Haihan Jiao, Alicia SL Lim, Therese E Fazio Coles, Rachel M McQuade, John B Furness, Holly R Chinnery
EXPERIMENTAL EYE RESEARCH | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2020
PURPOSE: The highly innervated cornea is susceptible to nerve loss secondary to systemic diseases such as diabetes and metabolic disturbances caused by high-fat diet. In this study, we characterize the effect of high-fat diet on the mouse corneal neuroimmune phenotype, including changes to corneal nerve density and resident immune cells, alongside the clinical assessment of corneal thickness and endothelial cell density. METHODS: Male C57Bl6/J mice, aged 10 weeks, were fed a high-fat diet (60 kcal% fat, 5.2 kcal/g) or control diet (10 kcal%, 3.8 kcal/g) for 16 weeks. At the study endpoint, metabolic parameters (HbA1c, weight, fasting glucose, body fat) were measured to confirm metabolic dist..View full abstract
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Awarded by National Health and Medical Research Council Project Grant (Australia)
The authors thank Enie Lei, Billie Hunne and Ada Koo from the Furness laboratory for their assistance with tissue collection and processing. The authors acknowledge the Florey Advanced Microscopy Facility at the Florey Institute of Neuroscience & Mental Health Facility for provision of instrumentation, training and general support. This research was supported by the National Health and Medical Research Council Project Grant (Australia) (HRC; APP1126540).