Genes regulating membrane-associated E-cadherin and proliferation in adenomatous polyposis coli mutant colon cancer cells: High content siRNA screen
Lauren E King, Hui-Hua Zhang, Cathryn M Gould, Daniel W Thomas, Lachlan W Whitehead, Kaylene J Simpson, Antony W Burgess, Maree C Faux
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2020
Truncating mutations in the tumour suppressor gene APC occur frequently in colorectal cancers and result in the deregulation of Wnt signalling as well as changes in cell-cell adhesion. Using quantitative imaging based on the detection of membrane-associated E-cadherin, we undertook a protein coding genome-wide siRNA screen to identify genes that regulate cell surface E-cadherin in the APC-defective colorectal cancer cell line SW480. We identified a diverse set of regulators of E-cadherin that offer new insights into the regulation of cell-cell adhesion, junction formation and genes that regulate proliferation or survival of SW480 cells. Among the genes whose depletion promotes membrane-assoc..View full abstract
Awarded by National Health and Medical Research Council (NH&MRC) Australia Program
This work was supported by the Ludwig Institute for Cancer Research, the National Health and Medical Research Council (NH&MRC) Australia Program grant #487922 and the Walter and Eliza Hall Institute. The Victorian Centre for Functional Genomics (K.J.S.) is funded by the Australian Cancer Research Foundation (ACRF), the Australian Phenomics Network (APN) through funding from the Australian Government's National Collaborative Research Infrastructure Strategy (NCRIS) program and the Peter MacCallum Cancer Centre Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.