Journal article

Influenza-specific IgG1( ) memory B-cell numbers increase upon booster vaccination in healthy adults but not in patients with predominantly antibody deficiency

Gemma E Hartley, Emily SJ Edwards, Julian J Bosco, Samar Ojaimi, Robert G Stirling, Paul U Cameron, Katie Flanagan, Magdalena Plebanski, Philip Mark Hogarth, Robyn E O'Hehir, Menno C Zelm



Background: Annual influenza vaccination is recommended to all individuals over 6 months of age, including predominantly antibody deficiency (PAD) patients. Vaccination responses are typically evaluated by serology, and because PAD patients are by definition impaired in generating IgG and receive immunoglobulin replacement therapy (IgRT), it remains unclear whether they can mount an antigen-specific response. Objective: To quantify and characterise the antigen-specific memory B (Bmem) cell compartment in healthy controls and PAD patients following an influenza booster vaccination. Methods: Recombinant hemagglutinin (HA) from the A/Michigan/2015 H1N1 (AM15) strain with an AviTag was generated..

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Awarded by Australian National Health and Medical Research Council (NHMRC)

Funding Acknowledgements

We acknowledge the support of Dr Josh Chatelier with clinical data collection, Ms Pei Mun Aui, Dr Craig I McKenzie, Ms. Sandra Esparon and Dr Bruce Wines for technical assistance, Alfred Pathology and Medical Day Unit staff for blood sample collection, and AMREP Flow core facility for maintenance of the flow cytometers. This study was supported by a Senior Research Fellowship (1117687 to MCvZ) and a Project Grant (1141840 to MP, KA and MCvZ) from the Australian National Health and Medical Research Council (NHMRC), and by The Jeffrey Modell Foundation.