Mycoplasma bovis mbfN Encodes a Novel LRR Lipoprotein That Undergoes Proteolytic Processing and Binds Host Extracellular Matrix Components
James Y Adamu, Filimon Mitiku, Carol A Hartley, Fiona M Sansom, Marc S Marenda, Philip F Markham, Glenn F Browning, Kelly A Tivendale
Journal of Bacteriology | AMER SOC MICROBIOLOGY | Published : 2021
Mycoplasma bovis causes serious infections in ruminants, leading to huge economic losses. Lipoproteins are key components of the mycoplasma membrane and are believed to function in nutrient acquisition, adherence, enzymatic interactions with the host, and induction of the host's immune response to infection. Many genes of M. bovis have not been assigned functions, in part because of their low sequence similarity with other bacteria, making it difficult to extrapolate gene functions. This study examined functions of a surface-localized leucine-rich repeat (LRR) lipoprotein encoded by mbfN of M. bovis PG45. Homologs of MbfN were detected as 48-kDa peptides by Western blotting in all the strain..View full abstract
J.Y.A. and F.M. were supported by Melbourne International Fee Remission Scholarships and Melbourne International Research Scholarships from The University of Melbourne.