Journal article

A radiolabeled drug tracing method to study neurotrophin-3 retention and distribution in the cochlea after nano-based local delivery

Patrick Lam, Niliksha Gunewardene, Yutian Ma, Frank Caruso, Nguyen Trung, Brianna Flynn, Andrew K Wise, Rachael T Richardson

METHODSX | ELSEVIER | Published : 2020

Abstract

Hearing loss is the most common sensory deficit worldwide with no approved therapeutics for treatment. Local neurotrophin delivery into the cochlea has shown great potential in protecting and repairing the sensory cells important for hearing. However, delivery of these factors into the inner ear at therapeutic levels over a sustained period of time has remained a challenge restricting clinical translation. We have developed a method to test the pharmacokinetics of neurotrophin released from porous silica particles called 'supraparticles' that can provide sustained release of neurotrophins to the inner ear.•This report describes a radiolabeling method to examine neurotrophin retention and dis..

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Grants

Awarded by Department of Defense USA, Hearing Restoration Research Program, Translational Research Award


Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology


Awarded by NHMRC Senior Principal Research Fellowship


Funding Acknowledgements

1 This work was funded with support from the Department of Defense USA, Hearing Restoration Research Program, Translational Research Award W81XWH1810276, The Garnett Passe and Rodney Williams Memorial Foundation, the Australian National Health and Medical Research Council (NHMRC) (GNT 1142910), and the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036). F.C. acknowledges the award of an NHMRC Senior Principal Research Fellowship (GNT1135806). The Bionics Institute acknowledges the support it receives from the Victorian Government, Australia, through its Operational Infrastructure Support Program. The authors would like to thank Donna Tuari, James Firth, Sayward Barone, Alex Thompson, Ella Trang and Caitlin Singleton from the Bionics Institute, Melbourne, Australia, for technical assistance, Patricia WM Ho from St Vincent's Institute, Melbourne, Australia for the use of the gamma counter and David Casley from ProSearch International, Melbourne, Australia, for technical assistance with protein radiolabelling.