Proteomics reveals region-specific hemostatic alterations in response to mechanical ventilation in a preterm lamb model of lung injury
Christine Schmid, Vera Ignjatovic, Boyuan Pang, Shuai Nie, Nicholas A Williamson, David G Tingay, Prue M Pereira-Fantini
Thrombosis Research | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2020
INTRODUCTION: Preterm infants often require assisted ventilation, however ventilation when applied to the immature lung can initiate ventilator-induced lung injury (VILI). The biotrauma which underscores VILI is largely undefined, and is likely to involve vascular injury responses, including hemostasis. We aimed to use a ventilated, preterm lamb model to: (1) characterize regional alterations in hemostatic mediators within the lung and (2) assess the functional impact of protein alterations on hemostasis by analyzing temporal thrombin generation. MATERIALS AND METHODS: Preterm lambs delivered at 124 to 127 days gestation received 90 min of mechanical ventilation (positive end-expiratory pres..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) Centre of Research in Excellence
Awarded by NHMRC Clinical Career Development Fellowship
Awarded by National Health and Medical Research Council (NHMRC)
MCRI is supported by the Victorian Government's Operational Infrastructure Program. This publication has emanated from research supported in part by a research grants from National Health and Medical Research Council (NHMRC) Centre of Research in Excellence (GNT ID 1057514) and Project Grant (Grant ID 1009287). DGT was supported by a NHMRC Clinical Career Development Fellowship (Grant ID 1053889 and 1123859). Chiesi Farmaceutici S.p.A. provided the Curosurf used in this study as part of an unrestricted grant (D.G.T.) at the Murdoch Childrens Research Institute. Chiesi Farmaceutici and Swisstom had no involvement in study design, implementation, analysis, interpretation, or reporting.