On the utility of fluorescence-detection analytical ultracentrifugation in probing biomolecular interactions in complex solutions: a case study in milk
Jennifer M Crowther, Marita Broadhurst, Thomas M Laue, Geoffrey B Jameson, Alison J Hodgkinson, Renwick CJ Dobson
European Biophysics Journal | SPRINGER | Published : 2020
β-Lactoglobulin is the most abundant protein in the whey fraction of ruminant milks, yet is absent in human milk. It has been studied intensively due to its impact on the processing and allergenic properties of ruminant milk products. However, the physiological function of β-lactoglobulin remains unclear. Using the fluorescence-detection system within the analytical ultracentrifuge, we observed an interaction involving fluorescently labelled β-lactoglobulin in its native environment, i.e. cow and goat milk, for the first time. Co-elution experiments support that these β-lactoglobulin interactions occur naturally in milk and provide evidence that the interacting partners are immunoglobulins, ..View full abstract
We acknowledge the following for funding support, in part: (1) the New Zealand Ministry of Business, Innovation and Employment, (2) the New Zealand Royal Society Marsden Fund, (3) the Biomolecular Interaction Centre, University of Canterbury, and (4) The Riddet Institute.