Journal article

Grass-Specific EPAD1 Is Essential for Pollen Exine Patterning in Rice

HuanJun Li, Yu-Jin Kim, Liu Yang, Ze Liu, Jie Zhang, Haotian Shi, Guoqiang Huang, Staffan Persson, Dabing Zhang, Wanqi Liang

PLANT CELL | AMER SOC PLANT BIOLOGISTS | Published : 2020

Abstract

The highly variable and species-specific pollen surface patterns are formed by sporopollenin accumulation. The template for sporopollenin deposition and polymerization is the primexine that appears on the tetrad surface, but the mechanism(s) by which primexine guides exine patterning remain elusive. Here, we report that the Poaceae-specific EXINE PATTERN DESIGNER 1 (EPAD1), which encodes a nonspecific lipid transfer protein, is required for primexine integrity and pollen exine patterning in rice (Oryza sativa). Disruption of EPAD1 leads to abnormal exine pattern and complete male sterility, although sporopollenin biosynthesis is unaffected. EPAD1 is specifically expressed in male meiocytes, ..

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University of Melbourne Researchers

Grants

Awarded by National Key Technologies Research and Development Program of China


Awarded by National Natural Science Foundation of China (NSFC)


Awarded by Program of Introducing Talents of Discipline to Universities (grants 111 Project)


Awarded by DP


Awarded by Villum Investigator


Awarded by Novo Nordisk Laureate grant


Awarded by SJTU JiRLMDS Joint Research Fund


Funding Acknowledgements

We thank Zhijin Luo, Mingjiao Chen, and Zibo Chen (Shanghai Jiao Tong University) for mutant screening and generation of F2 populations for mapping; Yu Kong and Xu Wang (Electron Microscopy Facilities of Neuroscience, Chinese Academy of Science) for assistance with EM sample preparation and analysis; Natalie Betts (School of Agriculture, Food and Wine, University of Adelaide) for editing; and Hongyan Yao (State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China) for assisting protein-lipid overlay assays. This work was supported by funds from the National Key Technologies Research and Development Program of China (grant 2016YFD0100902); the National Natural Science Foundation of China (NSFC; grants U19A2031 and 31670309); and the China Innovative Research Team, Ministry of Education, and the Program of Introducing Talents of Discipline to Universities (grants 111 Project and B14016). Fundingwasalso provided byARCFT and DP(grants DP190101941 and FT160100218 toS.P.); Villum Investigator (grant 25915 to S.P.); a Novo Nordisk Laureate grant (NNF19OC0056076; to S.P.); and SJTU JiRLMDS Joint Research Fund (grant MDS-JF-2019A05 to S.P. and L.W.).