Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells.
Monica Nafria, Constanze Bonifer, Edouard Guy Stanley, Elizabeth Siewsun Ng, Andrew George Elefanty
STAR Protoc | Elsevier BV | Published : 2020
This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17+ hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45+CD34+RUNX1C+ progenitors share an accessible chromatin profile with adult hematopoietic stem cells and multipotent progenitors. Therefore, this protocol is suitable for the mechanistic study of human multipotent progenitor development and for modeling childhood leukemias. For complete details on th..View full abstract