Journal article

Human Insulin-like Peptide 5 (INSL5). Identification of a Simplified Version of Two-Chain Analog A13

Xiaozhou Zhang, Ross AD Bathgate, Mohammed Akhter Hossain

ACS MEDICINAL CHEMISTRY LETTERS | AMER CHEMICAL SOC | Published : 2020

Abstract

The receptor for insulin-like peptide 5 (INSL5), RXFP4, is a potential pharma target for treating human conditions such as constipation, anorexia, and obesity. However, since INSL5 has a complex structure of two chains and three disulfide bonds, its synthesis has proven to be extremely difficult via either chemical or recombinant approaches. Previous studies led to the engineering of a high yielding simplified INSL5 analog, named analog 13 (A13), which retains native INSL5-like activity. The focus of this study is to further simplify the structure of A13 by truncating the N-terminal residues of the B-chain. We have found that the first six residues at the N-terminus of A13 are not important ..

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Grants

Awarded by NHMRC (Australia) IDEAS grant


Awarded by NHMRC Research Fellowship


Funding Acknowledgements

This research was funded by NHMRC (Australia) IDEAS grant #APP1182996 to MAH. During these studies, RADB was the recipient of an NHMRC Research Fellowship (1135837). Studies at the Florey Institute were supported by the Victorian Government's Operational Infrastructure Support Program.