Liposomes loaded with transforming growth factor beta 1 promote odontogenic differentiation of dental pulp stem cells
Liming Jiang, Wayne Nishio Ayre, Genevieve E Melling, Bing Song, Xiaoqing Wei, Alastair James Sloan, Xu Chen
JOURNAL OF DENTISTRY | ELSEVIER SCI LTD | Published : 2020
OBJECTIVES: This study investigated whether novel liposome formulations loaded with transforming growth factor β1 (TGF-β1) could promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) for dentine-pulp regeneration. METHODS: 0-100 ng/mL of liposomal TGF-β1 was prepared using the thin-film hydration method. Release of TGF-β1 from the liposomes was quantified by an enzyme-linked immunosorbent assay (ELISA). The hDPSCs were treated with different concentrations of liposomal TGF-β1 and cell viability was tested using an MTT assay. "Osteodentine" differentiation capacity was assessed by RT-qPCR, ELISA and Alizarin red S staining. RESULTS: The ELISA results showed that lip..View full abstract
Awarded by National Science Foundation of China
This study was supported by grant from the National Science Foundation of China (No. 81500898) and Natural Science Foundation of Liaoning Province of China(No.2019-MS-387).).