Journal article

Hybrid 213 nm photodissociation of cationized Sterol lipid ions yield [M]( center dot) Radical products for improved structural characterization using multistage tandem mass spectrometry

Henry West, Gavin E Reid

ANALYTICA CHIMICA ACTA | ELSEVIER | Published : 2021

Abstract

Sterols are a class of lipid molecules that include cholesterol, oxysterols, and sterol esters. Sterol lipids play critical functional roles in mammalian biology, including the dynamic regulation of cell membrane fluidity, as precursors for the synthesis of bile acids, steroid hormones and vitamin D, as regulators of gene expression in lipid metabolism, and for cholesterol transport and storage. The most common method employed for sterol analysis is high performance liquid chromatography coupled with tandem mass spectrometry (MS/MS). However, conventional collision induced dissociation (CID) methods used for ion activation during MS/MS typically fail to provide sufficient structural informat..

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University of Melbourne Researchers