Journal article

Manganese import protects Salmonella enterica serovar Typhimurium against nitrosative stress

Shehla Yousuf, Joyce E Karlinsey, Stephanie L Neville, Christopher A McDevitt, Stephen J Libby, Ferric C Fang, Elaine R Frawley

Metallomics | ROYAL SOC CHEMISTRY | Published : 2020


Nitric oxide (NO˙) is a radical molecule produced by mammalian phagocytic cells as part of the innate immune response to bacterial pathogens. It exerts its antimicrobial activity in part by impairing the function of metalloproteins, particularly those containing iron and zinc cofactors. The pathogenic Gram-negative bacterium Salmonella enterica serovar typhimurium undergoes dynamic changes in its cellular content of the four most common metal cofactors following exposure to NO˙ stress. Zinc, iron and magnesium all decrease in response to NO˙ while cellular manganese increases significantly. Manganese acquisition is driven primarily by increased expression of the mntH and sitABCD transporters..

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Awarded by NIH

Awarded by NHMRC

Awarded by Australian Research Council

Funding Acknowledgements

We thank Ms. Sarah Hasty for technical assistance with ICP-MS sample preparation. S. Y. and E. R. F. were supported by startup funding from Rhodes College. J. E. K., S. J. L. and F. C. F. were supported by NIH AI112640 and AI118962. S. L. N. and C. A. M. were supported by NHMRC 1140554 and 1180826. S. L. N. was supported by NHMRC Early Career Research Fellowship 1142695. C. A. M. was supported by Australian Research Council Future Fellowship FT170100006.