Journal article

HBx-K130M/V131I Promotes Liver Cancer in Transgenic Mice via AKT/FOXO1 Signaling Pathway and Arachidonic Acid Metabolism

Amy P Chiu, Barbara R Tschida, Tung-Ting Sham, Lilian H Lo, Branden S Moriarity, Xiao-Xiao Li, Regina C Lo, David E Hinton, Dewi K Rowlands, Chi-On Chan, Daniel KW Mok, David A Largaespada, Nadia Warner, Vincent W Keng

MOLECULAR CANCER RESEARCH | AMER ASSOC CANCER RESEARCH | Published : 2019

Abstract

Chronic hepatitis B viral (HBV) infection remains a high underlying cause for hepatocellular carcinoma (HCC) worldwide, while the genetic mechanisms behind this remain unclear. This study elucidated the mechanisms contributing to tumor development induced by the HBV X (HBx) gene of predominantly Asian genotype B HBV and its common HBx variants. To compare the potential tumorigenic effects of K130M/V131I (Mut) and wild-type (WT) HBx on HCC, the Sleeping Beauty (SB) transposon system was used to deliver HBx Mut and WT into the livers of fumarylacetoacetate hydrolase (Fah)-deficient mice and in the context of transformation related protein 53 (Trp53) deficiency. From our results, HBx Mut had a ..

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University of Melbourne Researchers

Grants

Awarded by Shenzhen Science and Technology Innovation Commission


Awarded by Health Medical Research Fund


Awarded by NIH IMVTP


Awarded by General Research Fund of the Research Grant Council of the Hong Kong Special Administrative Region


Awarded by NSFC/RGC Joint Research Scheme


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This project was supported by The Shenzhen Science and Technology Innovation Commission (JCYJ20170413154748190, to V.W. Keng; JCYJ20160229173844278, to D.K.-W. Mok; and JCYJ20160330171116798, to C.-O. Chan), Health Medical Research Fund (11122171), Food and Health Bureau, and the Hong Kong SAR Government, NSFC/RGC Joint Research Scheme (N-PolyU503/16; to V. W. Keng) and the NIH IMVTP grant no. T32 AI083196-04 (to B.R. Tschida), General Research Fund of the Research Grant Council of the Hong Kong Special Administrative Region (Project no. 15302718), Hong Kong Chinese Materia Medica Standards Project (to D.K.-W. Mok), the Large Equipment Funds and University Research Facility in Chemical and Environmental Analysis and Life Sciences of the Hong Kong Polytechnic University.