RNA-eXpress annotates novel transcript features in RNA-seq data.
Samuel C Forster, Alexander M Finkel, Jodee A Gould, Paul J Hertzog
Bioinformatics | Published : 2013
Next-generation sequencing is rapidly becoming the approach of choice for transcriptional analysis experiments. Substantial advances have been achieved in computational approaches to support these technologies. These approaches typically rely on existing transcript annotations, introducing a bias towards known genes, require specific experimental design and computational resources, or focus only on identification of splice variants (ignoring other biologically relevant transcribed features contained within the data that may be important for downstream analysis). Biologically relevant transcribed features also include large and small non-coding RNA, new transcription start sites, alternative ..View full abstract