Journal article

Symbiosis, virulence and natural-product biosynthesis in entomopathogenic bacteria are regulated by a small RNA

Nick Neubacher, Nicholas J Tobias, Michaela Huber, Xiaofeng Cai, Timo Glatter, Sacha J Pidot, Timothy P Stinear, Anna Lena Luetticke, Kai Papenfort, Helge B Bode

Nature Microbiology | NATURE RESEARCH | Published : 2020


Photorhabdus and Xenorhabdus species have mutualistic associations with nematodes and an entomopathogenic stage1,2 in their life cycles. In both stages, numerous specialized metabolites are produced that have roles in symbiosis and virulence3,4. Although regulators have been implicated in the regulation of these specialized metabolites3,4, how small regulatory RNAs (sRNAs) are involved in this process is not clear. Here, we show that the Hfq-dependent sRNA, ArcZ, is required for specialized metabolite production in Photorhabdus and Xenorhabdus. We discovered that ArcZ directly base-pairs with the mRNA encoding HexA, which represses the expression of specialized metabolite gene clusters. In a..

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Awarded by DFG

Awarded by European Research Council

Funding Acknowledgements

This work was funded in part by the DFG (SFB 902, project no. B17) and the LOEWE Centre for Translational Biodiversity Genomics (LOEWE TBG), supported by the State of Hesse. K.P. acknowledges funding by the DFG (EXC 2051, grant no. 390713860), Vallee Foundation and European Research Council (grant no. StG-758212). We thank A. Goodman for providing pSAM-BT and for helpful discussions. We thank L. Poschel and A. K. Heinrich for the plasmid construction.