Journal article

Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase

Nora Y Hakami, Gregory J Dusting, Elsa C Chan, Manisha H Shah, Hitesh M Peshavariya

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2020

Abstract

Purpose: Corneal injury that occurs after burning with alkali initiates wound-healing processes, including inflammation, neovascularization, and fibrosis. Excessive reactions to injury can reduce corneal transparency and thereby compromise vision. The NADPH oxidase (Nox) enzyme complex is known to be involved in cell signaling for wound-healing angiogenesis, but its role in corneal neovascularization has been little studied. Methods: The center corneas of wild-type and Nox4 knockout (KO) mice were injured with 3 µL 1 M NaOH, while the contralateral corneas remained untouched. On day 7, mRNA expression levels of NADPH oxidase isoforms, the proangiogenic factors VEGF-A and TGFβ1, and proinflam..

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Grants

Awarded by Heart Foundation Postdoctoral Fellowship


Awarded by National Heart Foundation of Australia


Funding Acknowledgements

Supported by King Abdul Aziz University, Jeddah, Saudi Arabi (NYH); Heart Foundation Postdoctoral Fellowship (PF11M6093) (HMP); and NHMRC Principal Research Fellowship and the Wiseman Trust (gift to University of Melbourne) (GJD). Studies were also supported by the National Heart Foundation of Australia Grant-in-Aid (G12M6726) and by grants from the Ophthalmic Research Institute of Australia grant and the Eye Research Australia Foundation. The Centre for Eye Research Australia received Operational Infrastructure Support from the Victorian State Government.