Use of copy number variation polymorphism to assess cellular and cell-free DNA chimerism following transplantation

Abstract

A state of genetic chimerism is created by allogenic transplantation, the quality and quantity of which may have diagnostic significance. The monitoring of cellular macrochimerism following allogeneic hematopoietic cell transplantation (HCT) is standard of care. It provides an assessment of engraftment, prognostic information, and guides therapeutic interventions. Separately, the discovery of cell-free DNA (cfDNA) chimerism following solid organ transplantation has raised the possibility that increases in donor genotype graft-derived cfDNA may be biomarker for rejection. As a result of these and other developments, the clinical and research requirements for chimerism analysis in the transp..