Thesis / Dissertation

Computational biology methods for identifying leaderless secretory proteins in Arabidopsis thaliana and other plant species

Andrew Gregory Lonsdale, Anthony Bacic (ed.)

Published : 2019

Abstract

Leaderless secretory proteins (LSPs) are proteins that are secreted yet lack the classical canonical signal peptide sequences and, therefore, by definition are undergoing unconventional protein secretion (UPS). We cannot necessarily assume all such proteins found in plant secretomes are LSPs due to the high possibility of cellular contamination arising from cellular disruption, leading to a mix of LSPs and contaminant proteins that are both characterised by the absence of signal peptides. The aims of this Thesis are to use computational biology methods to identify LSPs. In Chapter 1 the current knowledge of secretory pathways (LSPs and UPS) in plants, motifs for secretion, the difficulties ..

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University of Melbourne Researchers