Optimising molecular rotors to AIE fluorophores for mitochondria uptake and retention
Tze Cin OwYong, Siyang Ding, Na Wu, Thomas Fellowes, Sijie Chen, Jonathan M White, Wallace WH Wong, Yuning Hong
Chemical Communications | ROYAL SOC CHEMISTRY | Published : 2020
Molecular rotors exhibit fluorescence enhancement in a confined environment and thus have been used extensively in biological imaging. However, many molecular rotors suffer from small Stokes shift and self-aggregation caused quenching. In this work, we have synthesised a series of red emissive molecular rotors based on cationic α-cyanostilbene. Profoundly enhanced aggregation-induced emission (AIE) properties and greatly widened Stokes shifts can be achieved by molecular engineering. With specificity to stain mitochondria, we demonstrate a simple approach to achieve cell uptake and retention upon tuning the pyridinium substituent of the dyes.
Related Projects (1)
Awarded by Australian Research Council
Awarded by Rebecca L. Cooper Medical Research Foundation
Awarded by Australia-China Science and Research Fund-Joint Research Centre for Personal Health Technologies
Awarded by WWHW (Australian Research Council)
We thank LIMS Bioimaging Platform, La Trobe University for the access to confocal microscope and the Bio21 Mass Spectrometry and Proteomics Facility for technical support and access to mass spectrometers. This work was supported by grants to Y. H. (Australian Research Council DE170100058, Rebecca L. Cooper Medical Research Foundation PG2018043, and Australia-China Science and Research Fund-Joint Research Centre for Personal Health Technologies ACSRF65777) and WWHW (Australian Research Council CE170100026).