Performance of Ag-ELISA in the diagnosis of Taenia solium cysticercosis in naturally infected pigs in Tanzania
Mwemezi L Kabululu, Maria V Johansen, James ED Mlangwa, Ernatus M Mkupasi, Uffe C Braae, Chiara Trevisan, Angela Colston, Claudia Cordel, Marshall W Lightowlers, Helena A Ngowi
Parasites and Vectors | BMC | Published : 2020
BACKGROUND: Taenia solium is a zoonotic parasite responsible for neurocysticercosis-a major cause of late-onset acquired epilepsy in humans. Lack of affordable, specific and sensitive diagnostic tools hampers control of the parasite. This study assessed the performance of an antigen detection enzyme-linked immunosorbent assay (Ag-ELISA) in the diagnosis of viable T. solium cysticercosis in naturally infected slaughter-age pigs in an endemic area in Tanzania. METHODS: A total of 350 pigs were bled before they were slaughtered and their carcases examined. Serum was analyzed for circulating antigens by using a monoclonal antibody-based B158/B60 Ag-ELISA. Each carcase was examined for the presen..View full abstract
Awarded by Bill & Melinda Gates Foundation
Awarded by Australian National Health and Medical Research Council
This publication is based on research funded in part by the Bill & Melinda Gates Foundation (Grant no. OPP1009497) and the UK Government through GALVmed. Funding to MWL is acknowledged from the Australian National Health and Medical Research Council, grant GTN1105448. The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the Bill & Melinda Gates Foundation, the UK Government nor the Australian National Health and Medical Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.