Journal article

Morphometric analysis of in vitro human crystalline lenses using digital shadow photogrammetry

Ashik Mohamed, Heather A Durkee, Siobhan Williams, Fabrice Manns, Arthur Ho, Jean-Marie A Parel, Robert C Augusteyn

Experimental Eye Research | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2021


There is a great need for accurate biometric data on human lenses. To meet this, a compact tabletop optical comparator, the minishadowgraph, was built for measuring isolated eye lens shape and dimensions while the lens was fully immersed in supporting medium. The instrument was based around a specially designed cell and an illumination system which permitted image recording in both sagittal and equatorial (coronal) directions. Data were acquired with a digital camera and analyzed using a specially written MATLAB program as well as by manual measurements in image analysis software. The possible effect of lens orientation and gravity on the dimensions was examined by measuring dimensions with ..

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Awarded by National Eye Institute

Funding Acknowledgements

This study has been supported by National Eye Institute Grants 2R01EY021834 and P30EY14801 (Center Grant); the Australian Federal Government CRC Scheme through the Vision Cooperative Research Centre; the Hyderabad Eye Research Foundation; Florida Lions Eye Bank and the Beauty of Sight Foundation; Drs KR Olsen and ME Hildebrandt; Drs R Urs and A Furtado; an unrestricted grant from Research to Prevent Blindness to the Department of Ophthalmology, University of Miami and the Henri and Flore Lesieur Foundation (J -M. Parel).