What Are We Missing by Using Hydrophilic Enrichment? Improving Bacterial Glycoproteome Coverage Using Total Proteome and FAIMS Analyses
Ameera Raudah Ahmad Izaham, Ching-Seng Ang, Shuai Nie, Lauren E Bird, Nicholas A Williamson, Nichollas E Scott
Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2021
Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes. Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides. Here, we investigate HILIC enrichment-independent approaches for the study of bacterial glycoproteomes. Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demons..View full abstract
Related Projects (1)
Awarded by National Health and Medical Research Council of Australia (NHMRC)
This work was supported by a National Health and Medical Research Council of Australia (NHMRC) project grant awarded to NES (APP1100164).