Journal article
Analysis of Flagellin-Specific Adaptive Immunity Reveals Links to Dysbiosis in Patients With Inflammatory Bowel Disease
L Cook, DJ Lisko, MQ Wong, RV Garcia, ME Himmel, EG Seidman, B Bressler, MK Levings, TS Steiner
Cellular and Molecular Gastroenterology and Hepatology | ELSEVIER INC | Published : 2020
Abstract
Background & Aims: Bacterial flagellin is an important antigen in inflammatory bowel disease, but the role of flagellin-specific CD4+ T cells in disease pathogenesis remains unclear. Also unknown is how changes in intestinal microbiome intersect with those in microbiota-specific CD4+ T cells. We aimed to quantify and characterize flagellin-specific CD4+ T cells in Crohn's disease (CD) and ulcerative colitis (UC) patients and study their relationship with intestinal microbiome diversity. Methods: Blood was collected from 3 cohorts that included CD patients, UC patients, and healthy controls. Flow cytometry analyzed CD4+ T cells specific for Lachnospiraceae-derived A4-Fla2 and Escherichia coli..
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Awarded by Merck
Funding Acknowledgements
Supported by the Broad Medical Research Program at the Crohn's & Colitis Foundation of America (IBD-0326 to M.K.L and T.S.S); a Canadian Institutes of Health Research team grant in Immunoregulation and Inflammatory Bowel Disease (E.G.S, M. K.L., and T.S.S.); a Canada Research Chair in Immune Mediated Gastrointestinal Disorders and the B Kaufman McGill Chair in Inflammatory Bowel Disease (E. G.S.); a Frederick Banting and Charles Best Canada Graduate Scholarship (M.E.H.); and the British Columbia Children's Hospital Research Institute Bertram Hoffmeister Postdoctoral Fellowship (L.C.).