An optimized method to measure human FOXP3( ) regulatory Tcells from multiple tissue types using mass cytometry
Nicholas AJ Dawson, Avery J Lam, Laura Cook, Romy E Hoeppli, Raewyn Broady, Anne M Pesenacker, Megan K Levings
European Journal of Immunology | WILEY | Published : 2018
This work was supported by a grant from the Leukemia Lymphoma Society of Canada (to R.B. and M.K.L.). N.A.J.D. and A.J.L. hold CIHR Doctoral Research Awards; L.C. holds a BC Children's Hospital Research Institute postdoctoral fellowship; A.M.P. holds a JDRF postdoctoral fellowship; R.E.H. and M.K.L. hold salary awards from BC Children's Hospital Research Institute. We thank Mike Williams at the University of British Columbia Antibody Lab (Ablab.ca), Andy Johnson and David Ko at the University of British Columbia and BC Cancer Agency Flow Cores, respectively, and Fluidigm for helpful discussions, technical support, and custom antibody conjugation. The authors thank volunteers, patients, and their parents for contribution of samples, as well as BC Children's Hospital staff who made this study possible. We specifically thank Drs. Kim Morishita, Lori Tucker, Andrew Campbell, and Pascal Lavoie. N.A.J.D. designed and conducted experiments, analyzed data, and wrote the manuscript. A.J.L. designed and conducted experiments, analyzed data, and critically reviewed the manuscript. L.C. designed and conducted experiments, critically reviewed the manuscript, and provided experimental design guidance. R.E.H. and A.M.P. contributed to experiment design, reviewed results, and critically reviewed the manuscript. R.B. secured funding and contributed to experimental design. M.K.L. secured funding, provided overall guidance for experimental design and interpretation, and wrote the manuscript.