Journal article

Detection and identification of polyaromatic hydrocarbons (PAHs) contamination in soil using intrinsic fluorescence

Farah Qazi, Esmaeil Shahsavari, Steven Prawer, Andrew S Ball, Snjezana Tomljenovic-Hanic

ENVIRONMENTAL POLLUTION | ELSEVIER SCI LTD | Published : 2021

Abstract

Polyaromatic hydrocarbons (PAHs), such as pyrene, benzo[a]pyrene, phenanthrene, and anthracene induce toxic, carcinogenic, and mutagenic effects on living organisms and are considered as primary pollutants. Traditional methods for their identification are often laborious and time-consuming and do not account for the heterogeneous nature of their distribution. Here we present confocal microscopy as a rapid and accurate technique for direct analysis of PAHs in soil samples without the complexity of sample pre-processing which might delay results for several days. The method uses the intrinsic fluorescence of PAHs for detection and their emission spectra for the identification of different PAHs..

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