Journal article

A custom amplicon sequencing approach to detect resistance associated mutations and sequence types in Mycoplasma genitalium

EL Plummer, GL Murray, K Bodiyabadu, J Su, SM Garland, CS Bradshaw, TRH Read, SN Tabrizi, JA Danielewski

Journal of Microbiological Methods | ELSEVIER | Published : 2020

Abstract

BACKGROUND: Mycoplasma genitalium resistance to antibiotic treatments is increasing, with very limited treatment alternatives on the horizon. Surveillance via sequencing of multiple M. genitalium loci would allow: monitoring of known antibiotic resistance mutations, associations between resistance/treatment failure and specific mutations, and strain typing for epidemiological purposes. In this study we assessed the performance of a custom amplicon sequencing approach, which negates the cost of library preparation for next generation sequencing. METHODS: Fifty-two M. genitalium positive samples (cervical, vaginal, anal and rectal swabs, and urine) were used. Three regions associated with M. g..

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Grants

Awarded by NHMRC


Funding Acknowledgements

We thank Jimmy Twin for his contribution in selecting the target sites and associated primers used for the multiplex amplicon sequence in this study. This study was funded through an NHMRC program grant (APP1071269) awarded to SMG.