Journal article
Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source
Haoyuan Li, Reza Nazari, Brian Abbey, Roberto Alvarez, Andrew Aquila, Kartik Ayyer, Anton Barty, Peter Berntsen, Johan Bielecki, Alberto Pietrini, Maximilian Bucher, Gabriella Carini, Henry N Chapman, Alice Contreras, Benedikt J Daurer, Hasan DeMirci, Leonie Fluckiger, Matthias Frank, Janos Hajdu, Max F Hantke Show all
Scientific Data | NATURE RESEARCH | Published : 2020
Abstract
Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energ..
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Awarded by U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences
Awarded by US Department of Energy, Office of Science, Basic Energy Sciences
Awarded by US National Science Foundation
Awarded by Cluster of Excellence "CUI: Advanced Imaging of Matter" of the Deutsche Forschungsgemeinschaft (DFG)
Awarded by NSF Science and Technology Center grant
Awarded by NIH
Awarded by Russian Science Foundation
Awarded by European Union
Awarded by European Regional Development Fund
Funding Acknowledgements
Use of the Linac Coherent Light Source, SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC0276SF00515. The research conducted at UWM was supported by the US Department of Energy, Office of Science, Basic Energy Sciences under award DE-SC0002164 (algorithm design and development), and by the US National Science Foundation under awards STC 1231306 (numerical trial models and data analysis) and 1551489 (underlying analytical models). This work was supported by the Cluster of Excellence "CUI: Advanced Imaging of Matter" of the Deutsche Forschungsgemeinschaft (DFG) -EXC 2056 -project ID 390715994. The US National Science Foundation Award 1231306. The NSF Science and Technology Center grant NSF-1231306 (Biology with X-ray Lasers, BioXFEL). NIH grant 5R01GM117342. Helmholtz Associations Initiative and Networking Fund and Russian Science Foundation (Grant No. HRSF-0002/18-41-06001). This project has recieved funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 701647. The Swedish Research Council (to J.H.), The Knut and Alice Wallenberg Foundation (to J.H.), The European Research Council (to J.H.), and the project "Structural dynamics of biomolecular systems (ELIBIO)" (NO. CZ.02.1.01/0.0/0.0/15_003/0000447) from the European Regional Development Fund (to JH). The project "Structural dynamics of biomolecular systems (ELIBIO)" (NO. CZ.02.1.01/0.0/0.0/15_003/0000447) from the European Regional Development Fund. The European Research Council-Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (AXSIS). The Australian Research Council Centre of Excellence in Advanced Molecular Imaging (AMI).