Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae

Stephanie L Neville; Bart A Eijkelkamp; Amber Lothian; James C Paton; Blaine R Roberts; Jason W Rosch; Christopher A McDevitt

Journal article

Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae

Stephanie L Neville, Bart A Eijkelkamp, Amber Lothian, James C Paton, Blaine R Roberts, Jason W Rosch, Christopher A McDevitt

Communications Biology | NATURE RESEARCH | Published : 2020

DOI: 10.1038/s42003-020-01417-y

Download PDF

Abstract

Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulation of metal ion homeostasis remain to be elucidated. Here, we used cadmium, a non-physiological metal ion, to investigate how the bacterial pathogen, Streptococcus pneumoniae, resists metal ion stress and dyshomeostasis. By combining transcriptomics, metabolomics and metalloproteomics, we reveal that cadmium stress dysregulates numerous essential cellular pathways including central carbon metabolism, lipid membrane biogenesis and homeostasis, and capsule production at the transcriptional and/or functional level. Despite the breadth of cellular pathways susceptibl..

View full abstract

University of Melbourne Researchers

Christopher McDevitt Author Microbiology and Immunology

Stephanie Neville Author Microbiology and Immunology

Related Projects (5)

HARNESSING THE CONSEQUENCES OF IMPAIRED MITOCHONDRIAL ENERGY METABOLISM TO TREAT THE SYMPTOMS OF AGE-RELATED NEURONAL DECLINE

Cognitive function and locomotor ability decline due to advanced age and disorders such as motor neuron disease, Alzheimer’s and Parkinson’s..

INVESTIGATING THE ANTIMICROBIAL ACTIVITY OF ZINC AT THE HOST-PNEUMOCOCCAL INTERFACE

Streptococcus pneumoniae is a human-only bacterium that is responsible for killing more than one million people every year. This project wil..

DEFINING THE ROLE OF ZINC AT THE HOST-PNEUMOCOCCAL INTERFACE

Streptococcus pneumoniae is the world’s foremost bacterial pathogen. In Australia, bacterial infections are responsible for more than 9000 d..

MOLECULAR INSIGHTS INTO BACTERIAL METAL ION HOMEOSTASIS AND TOXICITY

This project aims to measure bacterial cellular metal concentrations, elucidate mechanisms cells use to adapt to changing extracellular meta..

NEW MOLECULAR TOOLS TO STUDY THE MECHANISMS OF BACTERIAL METAL HOMEOSTASIS

This project aims to provide new insight into how metal ion uptake is regulated. It will precisely measure the cellular concentrations of me..

Grants

Awarded by National Health and Medical Research Council (NHMRC)

Awarded by Australian Research Council (ARC)

Awarded by NHMRC

Awarded by ARC

Awarded by ALSAC

Funding Acknowledgements

This work was supported by the National Health and Medical Research Council (NHMRC) Program Grant 1071659 to J.C.P., Project Grants 1080784 and 1122582 to C. A.M., and the Australian Research Council (ARC) Discovery Project Grant DP170102102 to J.C.P. and C.A.M. S.L.N. is an NHMRC Early Career Research Fellow (1142695) and C.A.M. is an ARC Future Fellow (FT170100006). J.W.R is supported by 1U01AI124302, 1RO1AI110618, and by ALSAC. B.R.R acknowledges receiving partial support from the NHMRC (1061550 & 1138673), and the Motor Neuron Disease Research Institute of Australia. We thank Prof. A.G. McEwan for critical reading and discussions, and the Melbourne Mass Spectrometry and Proteomics Facility of the Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, mass spectrometry analysis support.