Journal article

Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae

Stephanie L Neville, Bart A Eijkelkamp, Amber Lothian, James C Paton, Blaine R Roberts, Jason W Rosch, Christopher A McDevitt



Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulation of metal ion homeostasis remain to be elucidated. Here, we used cadmium, a non-physiological metal ion, to investigate how the bacterial pathogen, Streptococcus pneumoniae, resists metal ion stress and dyshomeostasis. By combining transcriptomics, metabolomics and metalloproteomics, we reveal that cadmium stress dysregulates numerous essential cellular pathways including central carbon metabolism, lipid membrane biogenesis and homeostasis, and capsule production at the transcriptional and/or functional level. Despite the breadth of cellular pathways susceptibl..

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Awarded by National Health and Medical Research Council (NHMRC)

Awarded by Australian Research Council (ARC)

Awarded by NHMRC

Awarded by ARC

Awarded by ALSAC

Funding Acknowledgements

This work was supported by the National Health and Medical Research Council (NHMRC) Program Grant 1071659 to J.C.P., Project Grants 1080784 and 1122582 to C. A.M., and the Australian Research Council (ARC) Discovery Project Grant DP170102102 to J.C.P. and C.A.M. S.L.N. is an NHMRC Early Career Research Fellow (1142695) and C.A.M. is an ARC Future Fellow (FT170100006). J.W.R is supported by 1U01AI124302, 1RO1AI110618, and by ALSAC. B.R.R acknowledges receiving partial support from the NHMRC (1061550 & 1138673), and the Motor Neuron Disease Research Institute of Australia. We thank Prof. A.G. McEwan for critical reading and discussions, and the Melbourne Mass Spectrometry and Proteomics Facility of the Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, mass spectrometry analysis support.