Journal article

Critical cancer vulnerabilities identified by unbiased CRISPR/Cas9 screens inform on efficient cancer Immunotherapy

Margaret A Potts, Jackson A McDonald, Kate D Sutherland, Marco J Herold

European Journal of Immunology | WILEY | Published : 2020

Abstract

The mutational landscape of human cancers is highly complex. While next generation sequencing aims to comprehensively catalogue somatic alterations in tumor cells, it fails to delineate driver from passenger mutations. Functional genomic approaches, particularly CRISPR/Cas9, enable both gene discovery, and annotation of gene function. Indeed, recent CRISPR/Cas9 technologies have flourished with the development of more sophisticated and versatile platforms capable of gene knockouts to high throughput genome wide editing of a single nucleotide base. With new platforms constantly emerging, it can be challenging to navigate what CRISPR tools are available and how they can be effectively applied ..

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Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by Leukemia and Lymphoma Society of America


Awarded by Cancer Council of Victoria


Awarded by Victorian Cancer Agency Mid-Career Fellowship


Funding Acknowledgements

We thank all of our current and past colleagues at The Walter and Eliza Hall Institute and elsewhere. Our work was supported by grants and fellowships from the Australian National Health and Medical Research Council (NHMRC) (Project Grants 1186575 and 1145728,1143105,1159658 and Fellowship 1156095 to M.J.H.), the Leukemia and Lymphoma Society of America (LLS SCOR 7015-18 to M.J.H.), the Cancer Council of Victoria (project grant 1147328 and Venture Grant to M.J.H.), the Australian Phenomics Network (to M.J.H.). K.D.S. is supported by a Victorian Cancer Agency Mid-Career Fellowship (MCRF18003) and The Peter and Julie Alston Centenary Fellowship. M.A.P. and J.A.M. are recipients of the Research Training Scholarship, The University of Melbourne. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council Independent Research Institutes Infrastructure Support Scheme.