Journal article

Impact of gamma-chain cytokines on EBV-specific T cell cultures

Anna Merlo, Riccardo Turrini, Cristina Trento, Paola Zanovello, Riccardo Dolcetti, Antonio Rosato

Journal of Translational Medicine | BMC | Published : 2010


BACKGROUND: Recent preclinical adoptive immunotherapy studies in murine models prompt to employ "proper" rather than "as many as possible" antigen-specific T cells to gain better therapeutic results. Ideally, "proper" T cells are poorly differentiated in vitro, but retain the capacity to fully differentiate into effector cells in vivo, where they can undergo long-term survival and strong proliferation. Such requirements can be achieved by modifying culture conditions, namely using less "differentiating" cytokines than IL-2. METHODS: To evaluate this issue in human T cell cultures, we exploited a well characterized and clinical-grade protocol finalized at generating EBV-specific CTL for adopt..

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Awarded by Italian Ministry of Health

Awarded by European Community

Funding Acknowledgements

This study was partly supported by grants from the Italian Ministry of Health (Progetto oncologico di medicina molecolare: i tumori femminili; Progetto strategico: Farmaci cellulari, vaccini e bioterapie innovative dei tumori; Alleanza Contro il Cancro, ACC-4), the European Community (FP6 VITAL, Contract no. 037874) and the Italian Association for Cancer Research (AIRC).