Journal article

Impact of gamma-chain cytokines on EBV-specific T cell cultures

Anna Merlo, Riccardo Turrini, Cristina Trento, Paola Zanovello, Riccardo Dolcetti, Antonio Rosato

Journal of Translational Medicine | BMC | Published : 2010

Abstract

BACKGROUND: Recent preclinical adoptive immunotherapy studies in murine models prompt to employ "proper" rather than "as many as possible" antigen-specific T cells to gain better therapeutic results. Ideally, "proper" T cells are poorly differentiated in vitro, but retain the capacity to fully differentiate into effector cells in vivo, where they can undergo long-term survival and strong proliferation. Such requirements can be achieved by modifying culture conditions, namely using less "differentiating" cytokines than IL-2. METHODS: To evaluate this issue in human T cell cultures, we exploited a well characterized and clinical-grade protocol finalized at generating EBV-specific CTL for adopt..

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Grants

Awarded by Italian Ministry of Health


Awarded by European Community


Funding Acknowledgements

This study was partly supported by grants from the Italian Ministry of Health (Progetto oncologico di medicina molecolare: i tumori femminili; Progetto strategico: Farmaci cellulari, vaccini e bioterapie innovative dei tumori; Alleanza Contro il Cancro, ACC-4), the European Community (FP6 VITAL, Contract no. 037874) and the Italian Association for Cancer Research (AIRC).