Multiparametric Analyses of Human PBMCs Loaded Ex Vivo with a Candidate Idiotype Vaccine for HCV-Related Lymphoproliferative Disorders
Annacarmen Petrizzo, Maria Lina Tornesello, Maria Napolitano, Giovanna D'Alessio, Angelo Salomone Megna, Riccardo Dolcetti, Valli De Re, Ena Wang, Franco M Marincola, Franco M Buonaguro, Luigi Buonaguro
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2012
Hepatitis C virus (HCV) has been identified as one of the major risk factors for type II mixed cryoglobulinemia (MC), during the clinical evolution of chronic hepatitis, which may lead to development of B cell non-Hodgkin's lymphoma (NHL). We have previously shown that the candidate idiotype vaccine, based on the IGKV3-20 light chain protein, is able to induce activation and maturation of circulating antigen presenting cells (APCs) in both HCV-positive and HCV-negative healthy control subjects, with production of Th2-type cytokines. Here, the effect of the recombinant IGKV3-20 protein on human peripheral blood mononuclear cells (PBMCs) from HCV-positive subjects, with known blood levels of c..View full abstract
Awarded by Italian Ministry of Research
Awarded by Associazione Italiana per la ricerca sul Cancro
The study was funded by Italian Ministry of Research through the project "Alleanza Contro il Cancro'' (Contract No. ACC 4). AP was a Ph.D. student at University of Udine "Biomedical Science and Biotechnology'' and the recipient of a fellowship granted through the project. The work was partly supported by a grant from the Associazione Italiana per la ricerca sul Cancro (contract 10301 to RD) and the Fondazione Federica per la Vita (to RD). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.