Journal article

Prevalence of Borrelia Burgdorferi Infection in a Series of 98 Primary Cutaneous Lymphomas

Maurilio Ponzoni, Andres JM Ferreri, Silvia Mappa, Elisa Pasini, Silvia Govi, Fabio Facchetti, Daniele Fanoni, Alessandra Tucci, Arianna Vino, Claudio Doglioni, Emilio Berti, Riccardo Dolcetti



Borrelia burgdorferi has been variably associated with different forms of primary cutaneous lymphoma. Differences in prevalence rates among reported studies could be a result of geographic variability or heterogeneity in the molecular approaches that have been employed. In the present study, we investigated the prevalence of Borrelia burgdorferi sensu lato DNA in diagnostic tissue samples from fresh cutaneous biopsies of 98 primary cutaneous lymphomas and 19 normal skin controls. Three different polymerase chain reaction (PCR) protocols targeting the hbb, flagellin, and Osp-A genes were used. Direct sequencing of both sense and antisense strands of purified PCR products confirmed the specifi..

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