Journal article

UNCOUPLING OF GROWTH-INHIBITION AND DIFFERENTIATION IN DEXAMETHASONE-TREATED HUMAN RHABDOMYOSARCOMA CELLS

C DEGIOVANNI, PL LOLLINI, R DOLCETTI, L LANDUZZI, G NICOLETTI, E DANDREA, K SCOTLAND, P NANNI

British Journal of Cancer | NATURE PUBLISHING GROUP | Published : 1993

Abstract

The effects of dexamethasone, a synthetic glucocorticoid, and of N,N-dimethylformamide on in vitro growth and differentiation and on proto-oncogene expression of human rhabdomyosarcoma cells were studied. RD/18 clone cells (derived from the embryonal rhabdomyosarcoma cell line RD) treated with 100 nM dexamethasone showed an almost complete block of differentiation: about 5% myosin-positive cells were observed after 2 weeks of culture in dexamethasone-supplemented differentiation medium, compared to 20% of untreated cultures. Dexamethasone also induced a 20-30% growth inhibition and a more flattened morphology. The treatment with N,N-dimethylformamide induced a significantly increased proport..

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