In-depth analysis of compartmentalization of HIV-1 matrix protein p17 in PBMC and plasma
Marina Selleri, Riccardo Dolcetti, Francesca Caccurid, Emanuela Giombini, Gabriella Rozera, Isabella Abbate, Alessia Mammone, Stefania Zanussi, Debora Martorelli, Simona Fiorentini, Arnaldo Caruso, Maria Rosaria Capobianchi
NEW MICROBIOLOGICA | EDIZIONI INT SRL | Published : 2017
HIV-1 p17 plays an important role in the virus life-cycle and disease pathogenesis. Recent studies indicated a high heterogeneity of p17. A high number of insertions in the p17 carboxy-terminal region have been more frequently detected in patients with non-Hodgkin lymphoma (NHL), suggesting a role of altered p17 in lymphomagenesis. Based on p17 heterogeneity, possible PBMC/plasma compartmentalization of p17 variants was explored by ultra-deep pyrosequencing in five NHL patients. The high variability of p17 with insertions at the carboxy-terminal region was confirmed in plasma and observed for the first time in proviral genomes. Quasispecies compartmentalization was evident in 4/5 patients. F..View full abstract
Supported by the Associazione Italiana per la Ricerca sul Cancro; the Italian Ministry of Health, Programma di Ricerca Corrente e Finalizzata and the Italian Ministry of Health, Programma di Ricerca di Rilevante Interesse Nazionale, AIDS Grant.