Journal article

Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis

Laura Ceballos-Laita, Elain Gutierrez-Carbonell, Daisuke Takahashi, Andrew Lonsdale, Anunciacion Abadia, Monika S Doblin, Antony Bacic, Matsuo Uemura, Javier Abadia, Ana Flor Lopez-Millan

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | MDPI | Published : 2020

Abstract

Metal toxicity is a common problem in crop species worldwide. Some metals are naturally toxic, whereas others such as manganese (Mn) are essential micro-nutrients for plant growth but can become toxic when in excess. Changes in the composition of the xylem sap, which is the main pathway for ion transport within the plant, is therefore vital to understanding the plant's response(s) to metal toxicity. In this study we have assessed the effects of exposure of tomato roots to excess Mn on the protein profile of the xylem sap, using a shotgun proteomics approach. Plants were grown in nutrient solution using 4.6 and 300 µM MnCl2 as control and excess Mn treatments, respectively. This approach yiel..

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Grants

Awarded by Spanish State Research Agency (AEI) - European Regional Development Fund (FEDER) (AEI/FEDER, UE)


Awarded by Aragon Government


Awarded by JSPS KAKENHI


Funding Acknowledgements

Supported by the Spanish State Research Agency (AEI) co-financed by the European Regional Development Fund (FEDER) (project AGL2016-75226-R; AEI/FEDER, UE) and the Aragon Government (group A09-20R). Research conducted in Iwate University was in part supported by JSPS KAKENHI Grant Numbers 24-7373, 22120003, and 24370018. E.G.-C. was supported by a JAE Pre-CSIC contract and L.C.-L was supported by a FPI-MINECO contract.