Effects of excess manganese on the xylem sap protein profile of tomato (Solanum lycopersicum) as revealed by shotgun proteomic analysis

Abstract

Metal toxicity is a common problem in crop species worldwide. Some metals are naturally toxic, whereas others such as manganese (Mn) are essential micro‐nutrients for plant growth but can become toxic when in excess. Changes in the composition of the xylem sap, which is the main pathway for ion transport within the plant, is therefore vital to understanding the plant’s response(s) to metal toxicity. In this study we have assessed the effects of exposure of tomato roots to excess Mn on the protein profile of the xylem sap, using a shotgun proteomics approach. Plants were grown in nutrient solution using 4.6 and 300 μM MnCl2 as control and excess Mn treatments, respectively. This approach yiel..